Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins.
نویسندگان
چکیده
Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 1258 شماره
صفحات -
تاریخ انتشار 2015